Publications
Peer-reviewed papers and Book chapters
L. Barreiros, J. L. Amoreira, S. Machado, S. R. Fernandes, E. M. P. Silva, P. Sá, S. Kietaibl, M. A. Segundo
R. Nunes, F. Araújo, L. Barreiros, L. Bártolo, M. A. Segundo, N. Taveira, B. Sarmento, J. Das Neves
ACS Applied Materials and Interfaces 10(41), 34942-34953
Machado, S., Fernandes, S.R., Chaves, L.L., Lima, S. A. C., Silva, E. M. P., Barreiros, L., Reis, S., Segundo, M.A.
Journal of Separation Science 41(17), 3382-3388
Determination of salivary cotinine as tobacco smoking biomarker
A. N. Ramdzan, M. I. G. S. Almeida, M. J. McCullough, M. A. Segundo, S. D. Kolev
TrAC - Trends in Analytical Chemistry 105, 89-97
Mineral composition of dry dog foods: impact on nutrition and potential toxicity
A. M. Pereira, E. Pinto, E. Matos, F. Castanheira, A. A. Almeida, C. S. Baptista, M. A. Segundo, A. J. M. Fonseca, A. R. J. Cabrita
Journal of Agricultural and Food Chemistry 66(29), 7822-7830
V. Springer, L. Barreiros, M. Avena, M. A. Segundo
Nanosystems as modulators of intestinal dapsone and clofazimine delivery
L. L. Chaves, S. A. Costa Lima, A. C. C. Vieira, L. Barreiros, B. Sarmento, S. Reis
Biomedicine and Pharmacotherapy 103, 1392-1396
P. S. Peixoto, E. M. P. Silva, M. V. Osório, L. Barreiros, J. L. F. C. Lima, M. A. Segundo
Analytical Methods 10(18), 2180-2186
Gas-phase structural characterization of neuropeptides Y Y1 receptor antagonists using mass spectrometry: orbitrap vs triple quadrupole
E. M. P. Silva, P. A. M. M. Varandas, T. Melo, C. Barros, I. S. Alencastre, L. Barreiros, P. Domingues, M. Lamghari, M. R. M. Domingues, M. A. Segundo
Journal of Pharmaceutical and Biomedical Analysis, 151, 227-234
P. S. Peixoto, I. V. Tóth, S. Machado, L. Barreiros, A. Machado, A. A. Bordalo, J. L. F. C. Lima, M. A. Segundo
Analytical Methods 10(7), 690-696
COVER FEATURE
L. L.Chaves, S. A. Costa Lima, A. C. C. Vieira, L. Barreiros, M. A. Segundo, D. Ferreira, B. Sarmento, S. Reis
European Journal of Pharmaceutical Sciences 112, 28-37
New insights into the anti-inflammatory and antioxidant properties of nitrated phospholipids
T. Melo, S. S. Marques, I. Ferreira, M. T. Cruz, P. Domingues, M. A. Segundo, M. R. M. Domingues
Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum
I. I. Ramos, L. M. Magalhães, L. Barreiros, S. Reis, J. L. F. C. Lima, M. A. Segundo
Analytical and Bioanalytical Chemistry, 410(3), 981-988
Topical co-delivery of methotrexate and etanercept using lipid nanoparticles: A targeted approach for psoriasis management
M. Ferreira, L. Barreiros, M. A. Segundo, T. Torres, M. Selores, S. A. C. Lima, S. Reis
Colloids and Surfaces B: Biointerfaces, 159, 23-29
Does ultrasound improve the activity of alpha amylase? A comparative study towards a tailor-made enzymatic hydrolysis of starch
H. M. Oliveira, V. S. Correia, M. A. Segundo, A. J. M. Fonseca, A. R. J. Cabrita
LWT - Food Science and Technology, 84, 674-685
Analytical methods for quantification of tranexamic acid in biological fluids: a review
E. M. P. Silva, L. Barreiros, P. Sá, C. Afonso, S. Kozek-Langenecker, M. A. Segundo
Microchemical Journal, 134, 333-342
Risk assessment and statistical analysis approach for design and optimization of pH-sensitive nanoparticles for improved oral delivery of dapsone
L. L. Chaves, S. A. Costa Lima, A. C. C.Vieira, L. Barreiros, M. A. Segundo, D. Ferreira, B. Sarmento, S. Reis
Nanomedicine, 12, 1975-1190
Evaluation of the joint effect of the incorporation of mechanically deboned meat and grape extract on the formulation of chicken nuggets
H. H. Tournour, L. Cunha, L. M. Magalhães, R. Costa-Lima, M. A. Segundo
Food Science and Technology International, 23, 328-337
Effect of "Touriga nacional" grape extract on characteristics of mechanically deboned chicken meat kept under frozen storage
H. H. Tournour, M. A. Segundo, L. M. Magalhães, A. S. G. Costa, L. Cunha
Journal of Food Process Engineering, 40, 3, e12434
Characterization of phospholipid nitroxidation by LC-MS in biomimetic models and in H9c2 Myoblast using a lipidiomic approach
T. Melo, P. Domingues, R. P. Carvalho, T. R. Rodrigues, H. Girão, M. A. Segundo, M. R. M. Domingues
Free Radicals in Biology and Medicine, 106, 219-227
Dynamic flow-through approach to evaluate readily bioaccessible antioxidants in solid food samples
M. A. Maia, T. R. P. Soares, A. I. P. Mota, M. Rosende, L. M. Magalhães, M. Miró, M. A. Segundo
Talanta, 166, 162-168
Cellular interactions of a lipid-based nanocarrier model with human keratinocytes: unravelling transport mechanisms
E. Silva, L. Barreiros, M. A. Segundo, S. A. C. Lima, S. Reis
Acta Biomaterialia, 53, 439–449
Development and validation of a liquid chromatography-MS/MS method for simultaneous quantification of tenofovir and efavirenz in biological tissues and fluids
L. Barreiros, C. Cunha-Reis, E. M. P. Silva, J. R. B. Carvalho, J. das Neves, B. Sarmento, M. A. Segundo
Journal of Pharmaceutical and Biomedical Analysis, 136, 120-125
Analytical features of diclofenac evaluation in water as a potential marker of anthropogenic pollution
M. V. Osório, S. Reis, J. L. F. C. Lima, M. A. Segundo
Current Pharmaceutical Analysis, 13, 39-47
2016
Nanoparticles-in-film for the combined vaginal delivery of anti-HIV microbiocide drugs
C. Cunha-Reis, A. Machado, L. Barreiros, F. Araújo, R. Nunes, V. Seabra, D. Ferreira, M. A. Segundo, B. Sarmento, J. Neves
Journal of Controlled Release, 243, 43-53
Automatização do tratamento da amostra através da técnica de injeção de partículas
M. A. Segundo
Scientia Chromatographica, 8, 181-187
Methotrexate loaded lipid nanoparticles for topical management of skin-related diseases: design, characterization and skin permeating potential
M. Ferreira, E. Silva, L. Barreiros, M. A. Segundo, S. A. C. Lima, S. Reis
International Journal of Pharmaceutics, 512, 14-21
Do cinnamylideneacetophenones have antioxidant properties and a protective effect toward the oxidation of phosphatidylcholines?
E. M. P. Silva, T. Melo, B. C. Sousa, D. I. S. P. Resende, L. M. Magalhães, M. A. Segundo, A. M. S. Silva, M. R. M. Domingues
European Journal of Medicinal Chemistry, 121, 331-337
Photocatalytic ozonation of urban wastewater and surface drinking water using immobilized TiO2 and LEDs: micropollutants, antibiotic resistance genes and estrogenic effects
N. F. F. Moreira, M. Sousa, G. Macedo, A. R. Ribeiro, L. Barreiros, J. L. Faria, M. F. R. Pereira, S. Castro-Silva, M. A. Segundo, C. M. Manaia, Olga. C. Nunes, A. M. T. Silva
Water Research, 94, 10-22
Analysis of 17-β-estradiol and 17-α-ethinylestradiol in biological and environmental matrices – a review
L. Barreiros, J. F. Queiroz, L. M. Magalhães, A. M. T. Silva, M. A. Segundo
Microchemical Journal, 126, 243-262
Programmable flow system for automation of oxygen radical absorbance capacity assay using pyrogallol red for estimation of antioxidant reactivity
I. I. Ramos, B. J. R. Gregório, L. Barreiros, L. M. Magalhães, I. V. Tóth, S. Reis, J. L. F. C. Lima, M. A. Segundo
Talanta, 150, 599-606
Nanoscale delivery of resveratrol towards enhancement of supplements and nutraceuticals
A. R. Neves, S. Martins, M. A. Segundo, S. Reis
Nutrients, 8, 131, 1-14
Recent advances on mass spectrometry analysis of nitrated phospholipids
T. Melo, P. Domingues, R. Ferreira, I. Milic, M. Fedorova, S. M. Santos, M. A. Segundo, M. R. M. Domingues
Analytical Chemistry, 88, 2622-2629
Fluoroquinolones and sulfonamides: features of their determination in waters
P. S. Peixoto, I. V. Tóth, M. A. Segundo, J. L. F. C. Lima
International Journal of Environmental Analytical Chemistry, 96, 185-202
Development and validation of HPLC method with fluorometric detection for quantification of bisnaphthalimidopropyldiaaminooctane in animal tissues following administration in polymeric nanoparticles
M. A. Segundo, V. L. R. G. Abreu, M. V. Osório, S. Nogueira, A. Cordeiro-da-Silva, S. A. C. Lima
Journal of Pharmaceutical and Biomedical Analysis, 120, 290-296
Assessment of riboflavin in milk and infant formula by molecular recognition solid-phase extraction and fluorometric detection
M. V. Osório, S. S. Marques, H. M. Oliveira, L. Barreiros, M. A. Segundo
Journal of Food Composition and Analysis, 45, 141-146
Determination of salivary cotinine through solid phase extraction using a bead-injection lab-on valve approach hyphenated to hydrophilic interaction liquid chromatography
A. N. Ramdzan, L. Barreiros, M. I. G. S. Almeida, S. D. Kolev, M. A. Segundo
Journal of Chromatography A, 1429, 284-291
Fully automated monitoring of drug permeation across a cell monolayer
L. Zelená, S. S. Marques, M. A. Segundo, M. Miró, P. Pávek, H. Sklenářová, P. Solich
Analytical and Bioanalytical Chemistry, 408, 971-981
Rapid assessment of bioactive phenolics and methylxanthines in spent coffee grounds by FT-NIR spectroscopy
L. M. Magalhães, S. Machado, M. A. Segundo, J. A. Lopes, R. N. M. J. Páscoa
Talanta, 147, 460-467
High sensitivity programmable flow method for assessment of total antioxidant capacity in biological samples
S. S. Marques, I. V. Tóth, L. M. Magalhães, S. Reis, M. A. Segundo
Microchemical Journal, 124, 261-266
Low pressure chromatographic method for online automatic evaluation of lipid nanoparticles permeation using Franz diffusion cell
A. C. Alves, I. I. Ramos, C. Nunes, L. M. Magalhães, H. Sklenarova, J. L. F. C. Lima, S. Reis, M. A. Segundo
Talanta, 146, 369-374
2015
Valorization of grape pomace: extraction of bioactive phenolics with antioxidant properties
H. H. Tournour, M. A. Segundo, L. M. Magalhães, L. Barreiros, J. Queiroz, L. Cunha
Industrial Crops and Products, 74, 397-406
Myoglobin microplate assay to evaluate prevention of protein peroxidation
S. S. Marques, L. M. Magalhães, A. I. P. Mota, T. R. P. Soares, B. Korsak, S. Reis, M. A. Segundo
Journal of Pharmaceutical and Biomedical Analysis, 114, 305-311
Development and validation of HPLC method using a monolithic column for quantification of trans-resveratrol in lipid nanoparticles for intestinal permeability studies
A. R. Neves, S. Reis, M. A. Segundo
Journal of Agricultural and Food Chemistry, 63, 3114-3120
in collaboration with Biophysics and Biomembranes Unit
Value adding to red grape pomace exploiting eco-friendly FT-NIR spectroscopy technique
R. N. M. J. Páscoa, S. Machado, L. M. Magalhães, J. A. Lopes
Food and Bioprocess Tecnology, 8, 865-874
in collaboration with Chemometrics & Process Analytical Technology Unit
2014
A new topical therapy for psoriasis: development of methotrexate-loaded nanostructured lipid carriers
M. F. Pinto, C. Moura, C. Nunes, M. A. Segundo, S. A. C. Lima, S. Reis
International Journal of Pharmaceutics, 477, 519-526
in collaboration with Biophysics and Biomembranes Unit
Co-association of methotrexate and SPIONs into anti-CD64 antibody-conjugated PLGA nanoparticles for a theranostic application
C. C. Moura, M. A. Segundo, J. Neves, S. Reis, B. Sarmento
International Journal of Nanomedicine, 9, 4911-4922
in collaboration with Biophysics and Biomembranes Unit
Assessing oral bioaccessibility of trace elements in soils under worst-case scenarios by automated in-line dynamic extraction as a front end to inductively coupled plasma spectrometry
M. Rosende, L. M. Magalhães, M. A. Segundo, M. Miró
Analytica Chimica Acta, 842, 1-10
in collaboration with FI-TRACE group
COVER FEATURE
Insights on antioxidant assays for biological samples based on the reduction of copper complexes - The importance of analytical conditions
S. S. Marques, L. M. Magalhães, I. V. Tóth, M. A. Segundo
International Journal of Molecular Sciences, 15, 11387-11402
Lab-on-valve combined to kinetic-matching approach for fast evaluation of total antioxidant capacity in wines
I. I. Ramos, M. A. Maia, S. Reis, L. M. Magalhães, M. A. Segundo
Analytical Methods, 6, 3622–3628
COVER FEATURE
Lipid nanoparticles for topical and transdermal application for alopecia treatment: development, physicochemical characterization and in vitro release and penetration studies
M. J. Gomes, S. Martins, D. Ferreira, M. A. Segundo, S. Reis
International Journal of Nanomedicine, 9, 1231–1242
in collaboration with Biophysics and Biomembranes Unit
Kinetic matching approach applied to ABTS assay for high-throughput determination of total antioxidant capacity of food products
L. M. Magalhães, L. Barreiros, S. Reis, M. A. Segundo
Journal of Composition and Food Analysis, 33, 187–194
Antioxidant profile of commercial enological tannins determined by multiple chemical assays
L. M. Magalhães, I. I. Ramos, S. Reis, M. A. Segundo
Australian Journal of Grape and Wine Research, 20, 72–79
2013
Photosensitized oxidation of phosphatidylethanolamines monitored by electrospray tandem mass spectrometry
T. Melo, N. Santos, E. Alves, E. Maciel, M. A. F. Faustino, J. P. C. Tomé, M. G. P. M. S. Neves, A. Cunha, A. Almeida, P. Domingues, M. A. Segundo, M. R. M. Domingues
Journal of Mass Spectrometry, 48, 1357–1365
in collaboration with Mass Spectrometry Centre - U.Aveiro
Automated microdialysis-based system for in-situ microsampling and investigation of lead bioavailability in terrestrial environments under physiologically-based extraction conditions
M. Rosende, L. M. Magalhães, M. A. Segundo, M. Miró
Environmental Science and Tecnology, 47, 11668-11675
in collaboration with FI-TRACE group
Combining ultrasound assisted extraction and a microliter colorimetric assay for the streamlined and environmentally friendly determination of urea in animal feedstuff
H. M. Oliveira, M. A. Segundo, A. J. M. Fonseca, A. R. J. Cabrita
Journal of Agricultural and Food Chemistry, 61, 9602-9608
in collaboration with Animal Nutrition Unit
Insights about α-tocopherol and Trolox interaction with phosphatidylcholine monolayers under peroxidation conditions through Brewster Angle Microscopy
C. M. Castro, M. Pinheiro, M. Lúcio, J. J. Giner-Casares, L. Camacho, J. L. F. C. Lima, S. Reis, M. A. Segundo
Colloids and Surfaces B: Biointerfaces, 111, 626–635
in collaboration with Biophysics and Biomembranes Unit
FT-NIR spectroscopy as a tool for valorization of spent coffee grounds: Application to assessment of antioxidant properties
R. N. M. J. Pascoa, L. M. Magalhães, J. A. Lopes
Food Research International, 51, 579-586
in collaboration with Chemometrics & Process Analytical Technology Unit
2012
Standardization of antioxidant capacity assessment through microchemical methods using a kinetic matching approach
L. M. Magalhães, L. Barreiros, M. A. Maia, S. Reis, J. L. F. C. Lima, M. A. Segundo
Talanta, 97, 473-483 in collaboration with Biophysics and Biomembranes Unit
Automated solid-phase spectrophotometric system for optosensing of bromate in drinking waters
S. M. Oliveira, H. M. Oliveira, M. A. Segundo, A. O. S. S. Rangel, J. L. F. C. Lima, V. Cerdà
Analytical Methods, 4, 1229-1236
Automatic aluminum chloride method for routine estimation of total flavonoids in red wines and teas
L. M. Magalhães, M. I. G. S. Almeida, L. Barreiros, M. A. Segundo, S. Reis
Food Analytical Methods, 5, 530-539 in collaboration with Biophysics and Biomembranes Unit
2011
Automatic flow system for evaluation of polystyrene-divinylbenzene sorbents applied to preconcentration of phenolic pollutants
H. M. Oliveira, M. A. Segundo, J. L. F. C. Lima
International Journal of Environmental and Analytical Chemistry, 91, 884-899
Flow-through carbon nanofiber-based microsolid-phase extraction coupled to liquid chromatography for automatic determination of trace levels of priority herbicides in environmental matrices
W. Boonjob, M. Miró, M. A. Segundo, V. Cerdà
Analytical Chemistry, 83, 5237-5244
A membraneless gas-diffusion unit - multisyringe flow injection spectrophotometric method for ammonium determination in untreated environmental samples
M. I. G. S. Almeida, J. M. Estela, M. A. Segundo, V. Cerdà
Highly integrated flow assembly for automated dynamic extraction and determination of readily bioaccessible chromium(VI) in soils exploiting carbon nanostructure-based solid-phase extraction
M. Rosende, M. Miró, M. A. Segundo, J. L. F. C. Lima, V. Cerdà
Analytical and Bioanalytical Chemistry, 400, 2217-2227
CUPRAC-FIA system for automatic determination of total antioxidant capacity in biological samples
J. P. N. Ribeiro, L. M. Magalhães, M. A. Segundo, S. Reis, J. L. F. C. Lima
Analytical Sciences, 27, 483-488
in collaboration with Biophysics and Biomembranes Unit
Universal approach for mesofluidic handling of bead suspensions in lab-on-valve format
H. M. Oliveira, M. Miró, M. A. Segundo, J. L. F. C. Lima
Sequential injection enzyme based assays: a review
C. I. C. Silvestre, P. C. A. G. Pinto, M. A. Segundo, M. L. M. F. S. Saraiva, J. L. F. C. Lima
Analytica Chimica Acta, 689, 160-177
Spectrophotometric determination of bromate in water using multisyringe flow injection analysis
S. M. Oliveira, M. A. Segundo, A. O. S. S. Rangel, J. L. F. C. Lima, V. Cerdà
Analytical Letters, 44, 284-297
Analytical potential of the mesofluidic Lab-on-a-Valve platform as a front-end to column separation systems
M. Miró, H. M. Oliveira, M. A. Segundo
2010
Oscillating chemiluminescence systems: state of the art
M. Iranifam, M. A. Segundo, J. L. M. Santos, José, L. F. C. Lima, M. H. Sorouraddin
Luminescence, 25, 409-418
Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity
L. M. Magalhães, F. Santos, M. A. Segundo, S. Reis, J. L. F. C. Lima
Talanta, 83, 441-447
High-throughput microplate assay for the determination of drug partition coefficients
L. M. Magalhães, C. Nunes, M. Lúcio, M. A. Segundo, S. Reis, J. L. F. C. Lima
Nature Protocols, 5, 1823-1830
in collaboration with Biophysics and Biomembranes Unit
Fully automatic flow method for the determination of scavenging capacity against nitric oxide radical
J. P. N. Ribeiro, L. M. Magalhães, M. A. Segundo, S. Reis, J. L. F. C. Lima
Analytical and Bioanalytical Chemistry, 397, 3005-3014
in collaboration with Biophysics and Biomembranes Unit
Hydrogen peroxide, antioxidant compounds and biological targets: an in vitro scavenging approach using a MSFIA-fluorimetric system
J. P. N. Ribeiro, L. M. Magalhães, M. A. Segundo, S. Reis, J. L. F. C. Lima
Talanta, 81, 1840-1846
in collaboration with Biophysics and Biomembranes Unit
On-line renewable solid phase extraction hyphenated to liquid chromatography for the determination of UV filters using bead injection and multisyringe-lab-on-valve approach
H. M. Oliveira, M. A. Segundo, J. L. F. C. Lima, M. Miró, V. Cerdà
Journal of Chromatography A, 1217, 3575-3582
Online hyphenation of multimodal microsolid phase extraction involving renewable molecularly imprinted and reversed-phase sorbents to liquid chromatography for automatic multiresidue assays
W. Bonjoob, Y. Yu, M. Miró, M. A. Segundo, J. Wang, V. Cerdà,
Analytical Chemistry, 82, 3052-3050
Exploiting automatic on-line renewable molecularly-imprinted solid-phase extraction in lab-on-valve format as front end to liquid chromatography: application to the analysis of riboflavin in foodstuffs
H. M. Oliveira, M. A. Segundo, J. L. F. C. Lima, M. Miró, V. Cerdà
Analytical and Bioanalytical Chemistry, 397, 77-86